Abstract:
This study presents the isolation, purification, and characterization of a novel glucose oxidase (GOX) enzyme from Penicillium canescens Tt42. Although glucose oxidase is a well-known enzyme with extensive applications in various industries, a gap remains in optimizing enzyme recovery from filamentous fungi. The primary aim was to develop an efficient purification process for GOX while maximizing its enzymatic yield and stability. The methodology involved cultivating P. canescens Tt42 under optimized conditions and using several cell disruption methods, including sonication, freeze-thaw, French press, and high-pressure cell disrupter (Z-Plus series) from Constant Systems. Among these, the high-pressure cell disrupter provided the highest enzyme release efficiency. Purification of GOX followed with ammonium sulfate precipitation, anion exchange chromatography, and size exclusion chromatography, achieving an 8.6-fold purification. Results showed that GOX from P. canescens Tt42 is a stable, dimeric enzyme with an optimal activity at 25-30°C and pH 7, exhibiting favorable kinetic properties. The main takeaway is that P. canescens Tt42 produces a GOX enzyme with potential industrial applicability due to its high yield and stability under specific conditions.
Conclusion on the Role of Constant Systems Cell Disruption equipment:
The Constant Systems high-pressure cell disrupter played a vital role in the efficient extraction of glucose oxidase from P. canescens Tt42 by achieving high levels of cell disruption and enzyme release. Its rapid processing and reproducible results were particularly advantageous for large-scale applications, enabling this study to obtain high enzyme yields with minimal sample handling time. This equipment’s performance underscored its value in enzyme recovery processes from filamentous fungi, setting a standard for cell lysis efficiency in studies requiring robust enzyme extraction.
