TECHNOLOGY
Our Processs
Our unique process is based on precise and consistent control of high pressure producing:
Instantaneous high pressure
The sample is momentarily trapped in the high-pressure cylinder and acting pressures instantly move from ambient to the set pressure.
Shear stresses
The sample rushes across the fixed orifice geometry and starts to enter the fixed orifice.
Acceleration
the sample is accelerated through the orifice up to speeds of 650 metres per second.
Depressurisation
Once the sample has left the orifice acting pressures instantly return to ambient.
05
Impact
The sample now collides with the cooled heat exchange surface (which can be as low as -15°C) in less than one second of the process beginning.
HOW WE DO IT
How do we control all these effects?
Put simply, we very accurately control the process pressure.
Several equipment suppliers offer high pressure processing but how accurate and consistent is the pressure that is applied to the sample?
The data chart shown opposite, shows recent testing of a well-known high pressure homogenizer. We found that the homogenizer processed < 30% of the sample within 10% of the set pressure. In simple terms that means over two thirds of the sample is being processed outwith 10% of the target pressure.
The same testing indicated that our unique and proprietary hydraulic design and fixed orifice geometry meant that 99% of the sample was processed within 1% of the set pressure. This ensures that the exact same conditions are applied to the sample throughout the process. So whether it’s a 0.5mL single sample or 150L per hour continuous flow processing we give you the control and accuracy that guarantees repeatable, scalable highly efficient cell lysis.
Our 4 decades of experience has allowed us to provide an insight on the following inefective methods of cell lysis
OSOne Shot Cell Disruptor
The OS is recommended for total volumes of up to 24mL which is ideal for those users who wish to upgrade from small bench-top techniques such as bead beating and sonication.
MCMulti Shot Cell Disruptor
The MC Cell Disruptor is the most versatile model for small volume processing offered by Constant Systems Limited. At approximately 500mm² the MC sits very comfortable on the benchtop but is best suited when used on its tailor made trolley.
CFContinuous Flow Cell Disruptor
The CF Cell Disruptor offers Continuous Flow Processing with two models, the CF1 and CF2. Both models offer the same process and differ only by process speed, the CF1 offers up to 6L per hour and the CF2 offers up to 24L per hour processing rates.
HFRHigh Flow Rate Cell Disruptor
The HFR is the ideal production model and offers the same lysis efficiency as the standard CSL systems across the full range of processing pressures yet can provide a vastly superior flow rate.
ChillersChillers
These chillers are ideal for use in various industries, including laboratories and manufacturing, where precise and consistent cooling is required.
