The Constant Systems CF-1 cell disruptor has been a regular and central part of the Chemical Biology Research Facility’s protein purification workflows at the University of Warwick for over a decade. Whether we are lysing small volumes of E.coli cultures using the one-shot methodology or larger cultures of fungal cells using the continuous flow module, the CF-1 has proven invaluable to our work for its ease of use and reliability. Compared to alternative cell lysis techniques available to us, such as sonication and chemical lysis, the cell disruptor has been demonstrably faster for high throughput sample preparation and given consistent and robust results confirmed by downstream analysis. This has given us peace of mind in optimising our procedures and allowed us to process a variety of cell lines confidently.
Overall, the CF-1 has been a fantastic addition to our laboratory and facilitated the characterisation and further investigation of countless enzymatic domains which we have published in numerous high impact journals. Throughout our usage of the equipment, the Constant Systems team have been excellent in their servicing and advice, and we are excited to continue working with them in the future.
Dr Munro Passmore research Fellow Dept Chemistry University of Warwick
Christopher D. Fage, Munro Passmore, Ben P. Tatman, Helen G. Smith, Xinyun Jian, Upeksha C. Dissanayake, Mia E. Foran, G. Andrés Cisneros, Gregory L. Challis, Józef R. Lewandowski, and Matthew Jenner, Molecular
